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English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism, fragmented and copied into stable ds-cDNA (blue). The ds-cDNA is sequenced using high-throughput, short-read sequencing methods. These sequences can then be aligned to a reference genome sequence to reconstruct which genome regions were being transcribed. This data can be used to annotate where expressed genes are, their relative expression levels, and any alternative splice variants.[1]
Date
Source Own work
Author Thomas Shafee
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Also in series:

  • RNA Microarray
    RNA Microarray
  • RNA-Seq
    RNA-Seq
  • SAGE
    SAGE

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  1. ↑ Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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5 Nhlangula 2016

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Sweswinyana12:20, 13 Nyenyenyani 2017Xifaniso lexi tsongahatiweke kusukela hi 12:20, 13 Nyenyenyani 20171,020 × 803 (246 KB)Evolution and evolvabilitytext as paths to avoid artefacts
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08:41, 10 Nyenyenyani 2017Xifaniso lexi tsongahatiweke kusukela hi 08:41, 10 Nyenyenyani 20171,020 × 803 (87 KB)Evolution and evolvabilityreverse order of fragmentation and reverse-transcription
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