File:Phage display.png
Size of this preview: 800 × 509 pixels. Other resolutions: 320 × 204 pixels | 640 × 407 pixels | 1,024 × 652 pixels | 1,280 × 814 pixels | 2,826 × 1,798 pixels.
kuleha na ku anama hixitalo (2,826 × 1,798 ku anama na leha hi ti phikisele, Vukulu bya fayili: 612 KB, muxaka waMIME: image/png)
Nkomiso
| Description |
English: Phage display cycle. 1) fusion proteins for a viral coat protein + the gene to be evolved (typically an antibody fragment) are expressed in bacteriophage. 2) the library of phage are washed over an immobilised target. 3) the remaining high-affinity binders are used to infect bacteria. 4) the genes encoding the high-affinity binders are isolated. 5) those genes may have random mutations introduced and used to perform another round of evolution. The selection and amplification steps can be performed multiple times at greater stringency to isolate higher-affinity binders.
Français : traduction à améliorer : Cycle d'évolution. 1) Protéines de fusion pour une protéine virale d'enveloppe + le gène devant évoluer (typiquement un fragment d'anticorps) sont exprimées dans le bactériophage. 2) La banque de phages est lavées sur une cible immobilisée. 3) les liants à haute affinité restants sont utilisés pour infecter les bactéries. 4) les gènes codant pour les liants à haute affinité sont isolés. 5) ces gènes peuvent provoquer des mutations aléatoires introduites et utilisées pour effectuer un autre cycle d'évolution. Les étapes de sélection et d’amplification peuvent être effectuées plusieurs fois avec une plus grande rigueur pour isoler des liants d’affinité plus élevée. |
| Date | |
| Source | Own work |
| Author | Thomas Shafee |
Nawu wo pfumelela
I, the copyright holder of this work, hereby publish it under the following license:
This file is licensed under the Creative Commons Attribution 4.0 International license.
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Matimu ya fayili
thlava eka siku/nkarhi leswaku u vona leswi fayili ayirixiswona hi knarhi walowo
| Siku/Nkarhi | Xifanisonyana | Mpimo | Mutirhisi | Nhlamulo | |
|---|---|---|---|---|---|
| Sweswinyana | 07:53, 22 Mudyaxihi 2019 | | 2,826 × 1,798 (612 KB) | Evolution and evolvability | minor optimisation of label positions |
| 05:29, 22 Mudyaxihi 2019 |  | 2,901 × 1,798 (585 KB) | Evolution and evolvability | User created page with UploadWizard |
Ntsengo wakutirhisiwa ka fiyili leyi
Kuhava tluka leri khwekelaka eka fayili leyi
Global file usage
The following other wikis use this file:
- Usage on da.wikipedia.org
- Usage on en.wikipedia.org
- Usage on fa.wikipedia.org
- Usage on sv.wikipedia.org
- Usage on tr.wikipedia.org
- Usage on www.wikidata.org
- Usage on zh.wikipedia.org
